Anti-Granzyme B/GZMB antibody

Cat.#: 101886

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Product Information

  • Product Name
    Anti-Granzyme B/GZMB antibody
  • Documents
  • Description
    Rabbit polyclonal to Granzyme B/GZMB
  • Tested applications
    ELISA, WB, IHC-P, IP
  • Species reactivity
    Human Granzyme B / GZMB
  • Alternative names
    CCP1 antibody; HLP antibody; CCPI antibody; CGL1 antibody; CSPB antibody; SECT antibody; CGL-1 antibody; CSP-B antibody; CTLA1 antibody; CTSGL1 antibody; GZB antibody; Ctla1 antibody; Ctla-1 antibody; AI553453 antibody; CCP-1/C11 antibody; AI553453 antibody; CCP1 antibody; CCP-1/C11 antibody; CCPI antibody; CGL1 antibody; CGL-1 antibody; CSPB antibody; CSP-B antibody; Ctla1 antibody; CTLA1 antibody; Ctla-1 antibody; CTSGL1 antibody; Gamb antibody; Granzyme B antibody; Granzyme B antibody; GZB antibody; GZMB antibody; HLP antibody; SECT antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    Produced in rabbits immunized with purified, recombinant Human Granzyme B (rh Granzyme B; NP_004122.1; Met 1-Tyr 247). Granzyme B specific IgG was purified by human Granzyme B affinity chromatography.
  • Clonality
    Polyclonal
  • Formulation
    0.2 μm filtered solution in PBS with 5% trehalose
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    WB: 5-10 μg/mL

    ELISA: 0.5-1.0 μg/mL

    This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human Granzyme B. The detection limit for Human Granzyme B is 0.00245 ng/well.

    IHC-P: 1-10 μg/mL

    IP: 1-4 μg/mg of lysate

  • Validations

    Granzyme B / GZMB Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry

    Granzyme B / GZMB Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry

    Immunochemical staining of human Granzyme B in human lymph node with rabbit polyclonal antibody (1µg/mL, formalin-fixed paraffin embedded sections).

    Granzyme B / GZMB Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot

    Granzyme B / GZMB Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot

    Granzyme B / GZMB Antibody, Rabbit PAb, Antigen Affinity Purified

    Granzyme B / GZMB Antibody, Rabbit PAb, Antigen Affinity Purified

  • Background
    Granzyme B, also known as GZMB, is the most prominent member of the granzyme family of cell death-inducing serine proteases expressed in the granules of cytotoxic T lymphocytes (CTLs) and NK cells. Granzyme B enters the target cells depending on another membrane-binding granule protein, perforin, results in the activation of effector caspases and mitochondrial depolarization through caspase-dependent and -independent pathways, and consequently induces rapid cell apoptosis. Over 30 substrates of GZMB have been identified including the key substrate caspase-3, ICAD and Bid. GZMB is suggested to protect the host by lysing cells bearing on their surface 'nonself' antigens such as bacterial and viral infected-cells and tumor cells, and accordingly plays an essential role in immunosurveillance.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"