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Product Name
Anti-GPR157 antibody
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Description
Rabbit polyclonal antibody to GPR157
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Tested applications
WB, IF
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Species reactivity
Human
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was the antiserum was produced against synthesized peptide derived from human gpr157. aa range:81-130
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Clonality
Polyclonal
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Formulation
Liquid, Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500-1:2000
IF: 1:100-1:500
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Validations
Fig1:; Western blot analysis of GPR157 on RAW264.7 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2:; Western blot analysis of lysates from NIH/3T3 cells, using GPR157 Antibody. The lane on the right is blocked with the synthesized peptide.
Fig3:; Immunofluorescence analysis of HUVEC cells, using GPR157 Antibody. The picture on the right is blocked with the synthesized peptide.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"