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Product Name
Anti-Gmnn antibody
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Description
Rabbit polyclonal antibody to Gmnn
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Tested applications
ICC, IHC-P, FC
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Species reactivity
Mouse
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Alternative names
Gem antibody; AW546347 antibody
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Isotype
IgG
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Preparation
This antigen of this antibody was recombinant protein with mouse geminin aa 70-206 / 206.
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Clonality
Polyclonal
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Formulation
Liquid, 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
IHC-P: 1:100-1:200
ICC: 1:200
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Validations
Fig1: ICC staining Geminin in F9 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
Fig2: ICC staining Geminin in D3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- Geminin rabbit polyclonal antibody.
- Background
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References
- Mouse geminin inhibits not only Cdt1-MCM6 interactions but also a novel intrinsic Cdt1 DNA binding activity. Yanagi K., Mizuno T., You Z., Hanaoka F. J. Biol. Chem. 277:40871-40880(2002)
- Structural basis for inhibition of the replication licensing factor Cdt1 by geminin. Lee C., Hong B., Choi J.M., Kim Y., Watanabe S., Ishimi Y., Enomoto T., Tada S., Kim Y., Cho Y. Nature 430:913-917(2004)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"