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Anti-GCGR antibody

Cat.#: 175455

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-GCGR antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to GCGR
  • Tested applications
    WB, IHC-P, FC
  • Species reactivity
    Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Guinea Pig
  • Alternative names
    GGR antibody; GL-R antibody; MVAH antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was klh conjugated synthetic peptide derived from human glucagon receptor 11-110/477
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • Storage instructions
    Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
  • Applications

    WB:1:500-2000

    IHC-P:1:400-800

    FC:1μg/Test

  • Validations

    Fig1: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

    Fig1: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

    Fig2: Paraformaldehyde-fixed, paraffin embedded (human stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

    Fig2: Paraformaldehyde-fixed, paraffin embedded (human stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

    Fig3: Blank control(blue): Molt-4 Cells(fixed with 2% paraformaldehyde (10 min)).; Primary Antibody: Rabbit Anti-Glucagon Receptor/AF647 Conjugated antibody (175455#/AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.

    Fig3: Blank control(blue): Molt-4 Cells(fixed with 2% paraformaldehyde (10 min)).; Primary Antibody: Rabbit Anti-Glucagon Receptor/AF647 Conjugated antibody (175455#/AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.

    Fig4: Sample: Cerebellum (Mouse) Lysate at 40 ug; Primary: Anti-Glucagon Receptor at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 51 kD; Observed band size: 51 kD

    Fig4: Sample: Cerebellum (Mouse) Lysate at 40 ug; Primary: Anti-Glucagon Receptor at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 51 kD; Observed band size: 51 kD

    Fig5: Sample:Kidney (Mouse) Lysate at 40 ug; Primary: Anti-Glucagon Receptor at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 51kD; Observed band size: 51kD

    Fig5: Sample:Kidney (Mouse) Lysate at 40 ug; Primary: Anti-Glucagon Receptor at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 51kD; Observed band size: 51kD

  • Background
    P47871

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"