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Product Name
Anti-Galectin 3 (4B2) Mouse antibody
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Description
Galectin 3 (4B2) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF, IHC-P
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
Antigen: Synthetic Peptide of Galectin-3
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Clonality
Monoclonal
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Formulation
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/2000
IHC: 1/200
IF: 1/100-200
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Validations
Western blot analysis of Hela, diluted at 1:3000.
IHC staining of Human thyroid tissue paraffin-embedded, diluted at 1:200.
Western blot detection of Galectin-3/LGALS3 in Jurkat,CHO-K1,3T3 cell lysates using Galectin-3/LGALS3 (4B2) Mouse mAb(1:1000 diluted).Predicted band size:26KDa.Observed band size:28KDa.
Immunofluorescence analysis of Human-lung-cancer tissue. 1,Galectin-3 Monoclonal Antibody(6G2)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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Background
Swiss-Prot Acc.P17931.Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis . In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.
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