Anti-Galectin 3 (4B2) Mouse antibody

Cat.#: 168150

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Product Information

  • Product Name
    Anti-Galectin 3 (4B2) Mouse antibody
  • Documents
  • Description
    Galectin 3 (4B2) Mouse monoclonal antibody
  • Tested applications
    WB, ICC/IF, IHC-P
  • Species reactivity
    Human
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Synthetic Peptide of Galectin-3
  • Clonality
    Monoclonal
  • Formulation
    PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/2000

    IHC: 1/200

    IF: 1/100-200

  • Validations

    Western blot analysis of Hela, diluted at 1:3000.

    Western blot analysis of Hela, diluted at 1:3000.

    IHC staining of Human thyroid tissue paraffin-embedded, diluted at 1:200.

    IHC staining of Human thyroid tissue paraffin-embedded, diluted at 1:200.

    Western blot detection of Galectin-3/LGALS3 in Jurkat,CHO-K1,3T3 cell lysates using Galectin-3/LGALS3 (4B2) Mouse mAb(1:1000 diluted).Predicted band size:26KDa.Observed band size:28KDa.

    Western blot detection of Galectin-3/LGALS3 in Jurkat,CHO-K1,3T3 cell lysates using Galectin-3/LGALS3 (4B2) Mouse mAb(1:1000 diluted).Predicted band size:26KDa.Observed band size:28KDa.

    Immunofluorescence analysis of Human-lung-cancer tissue. 1,Galectin-3 Monoclonal Antibody(6G2)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

    Immunofluorescence analysis of Human-lung-cancer tissue. 1,Galectin-3 Monoclonal Antibody(6G2)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

  • Background
    Swiss-Prot Acc.P17931.Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis . In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"