-
Product Name
Anti-FGF18 antibody
- Documents
-
Description
Rabbit polyclonal to FGF18
-
Tested applications
ELISA, WB, IP
-
Species reactivity
Mouse FGF18
-
Alternative names
D130055P09Rik antibody; Fgf18 antibody; FGF18 antibody; FGF-18 antibody; FGF-18 antibody; RP23-413O19.3 antibody; UNQ420/PRO856 antibody; ZFGF5 antibody; FGF-18 antibody; D130055P09Rik antibody; ZFGF5 antibody; FGF-18 antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
Produced in rabbits immunized with purified, recombinant Mouse FGF18 (rh FGF18; NP_032031.1; Met1-Gly207). FGF18 specific IgG was purified by Mouse FGF18 affinity chromatography.
-
Clonality
Polyclonal
-
Formulation
0.2 μm filtered solution in PBS
-
Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse FGF18. The detection limit for Mouse FGF18 is < 0.039 ng/well.
IP: 1-4 μg/mg of lysate
-
Validations
FGF18 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
FGF18 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
-
Background
Fibroblast growth factor 18 (FGF18) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. It has been shown in vitro that FGF18 is able to induce neurite outgrowth in PC12 cells. Studies of the similar proteins in mouse and chick suggested that this protein is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine. Experiment datas identified FGF18 as a selective ligand for FGFR3 in limb bud mesenchymal cells, which suppressed proliferation and promoted their differentiation and production of cartilage matrix. FGF18 appears to regulate cell proliferation and differentiation positively in osteogenesis and negatively in chondrogenesis.
-
References
- Ohbayashi N, et al. (2002) FGF18 is required for normal cell proliferation and differentiation during osteogenesis and chondrogenesis. Genes Dev. 16(7): 870-9.
- Davidson D, et al. (2005) Fibroblast growth factor (FGF) 18 signals through FGF receptor 3 to promote chondrogenesis. J Biol Chem. 280(21): 20509-15.
- Liu Z, et al. (2007) FGF18 is required for early chondrocyte proliferation, hypertrophy and vascular invasion of the growth plate. Dev Biol. 302(1): 80-91.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"