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Product Name
Anti-EpCAM/TROP-1/TACSTD1 antibody
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Description
Rabbit polyclonal to EpCAM/TROP-1/TACSTD1
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Cynomolgus EpCAM / TROP-1 / TACSTD1
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Alternative names
ESA antibody; KSA antibody; M4S1 antibody; MK-1 antibody; DIAR5 antibody; EGP-2 antibody; EGP40 antibody; KS1/4 antibody; MIC18 antibody; TROP1 antibody; EGP314 antibody; HNPCC8 antibody; TACSTD1 antibody; EGP antibody; Ly74 antibody; gp40 antibody; CD326 antibody; EGP-2 antibody; TROP1 antibody; Egp314 antibody; Ep-CAM antibody; EpCAM1 antibody; Tacsd1 antibody; GA733-2 antibody; Tacstd1 antibody; CD326 antibody; CO17-1A antibody; CO-17A antibody; EGP antibody; EGP antibody; EGP-2 antibody; EGP-2 antibody; Egp314 antibody; EGP34 antibody; EGP40 antibody; Epcam antibody; EPCAM antibody; Ep-CAM antibody; Ep-CAM antibody; ESA antibody; GA733-2 antibody; GA733-2 antibody; gp40 antibody; hEGP-2 antibody; KS1/4 antibody; KSA antibody; Ly74 antibody; M4S1 antibody; MIC18 antibody; MK-1 antibody; Tacsd1 antibody; TACST-1 antibody; Tacstd1 antibody; TACSTD1 antibody; TROP1 antibody; TROP1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Cynomolgus EpCAM / TROP-1 / TACSTD1 (rh EpCAM / TROP-1 / TACSTD1; EHH55554.1; Met1-Lys265). EpCAM / TROP-1 / TACSTD1 specific IgG was purified by Cynomolgus EpCAM / TROP-1 / TACSTD1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Cynomolgus EpCAM / TROP-1 / TACSTD1. The detection limit for Cynomolgus EpCAM / TROP-1 / TACSTD1 is < 0.039 ng/well.
IHC-P: 0.1-1 μg/ml
IP: 1-4 μg/mg of lysate
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Validations
EpCAM / TROP-1 / TACSTD1 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
EpCAM / TROP-1 / TACSTD1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
EpCAM / TROP-1 / TACSTD1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
EpCAM / TROP-1 / TACSTD1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Background
Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type â… transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.
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References
- Brunner A, et al. (2008) EpCAM is predominantly expressed in high grade and advanced stage urothelial carcinoma of the bladder. J Clin Pathol. 61(3):307-10.
- Trzpis M, et al. (2008) EpCAM in morphogenesis. Front Biosci. 13: 5050-5.
- Munz M, et al. (2009) The emerging role of EpCAM in cancer and stem cell signaling. Cancer Res. 69(14): 5627-9.
- Carpenter G, et al. (2009) EpCAM: another surface-to-nucleus missile. Cancer Cell. 15(3): 165-6.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"