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Product Name
Anti-EpCAM (1D5) Mouse antibody
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Description
EpCAM (1D5) Mouse monoclonal antibody
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Tested applications
WB, IP
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Species reactivity
Human
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Alternative names
17 1A; 323/A3; Adenocarcinoma associated antigen; Adenocarcinoma-associated antigen; Antigen identified by monoclonal antibody AUA1; AUA1; CD326; CD326 antigen; Cell surface glycoprotein Trop 1; Cell surface glycoprotein Trop 2; Cell surface glycoprotein antibody
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Isotype
IgG1
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Preparation
Antigen: Purified recombinant EpCAM protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
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Validations
Immunoprecipitation analysis of HCT116 and Hela cell lysates using EpCAM.
Western blot analysis of extracts from CHO-K1,CHO-K1 transfected by Flag-EpCAM, MCF7(EpCAM positive),HCT116(EpCAM positive),and Hela(EpCAM negative) cell lysates using EpCAM mouse mAb (1:1000 diluted).Predicted band size:39KDa.Observed band size:39/35KDa.
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Background
Swiss-Prot Acc.P16422.May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"