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Product Name
Anti-DCX antibody
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Description
Rabbit polyclonal to DCX
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Human DCX
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Alternative names
Dbct antibody; DC antibody; DBCN antibody; LISX antibody; SCLH antibody; XLIS antibody; DBCN antibody; Dbct antibody; DC antibody; doublecortex antibody; doublecortex antibody; doubling antibody; doubling antibody; lissencephalin-X antibody; lissencephalin-X antibody; lissencephaly antibody; LISX antibody; lis-X antibody; lis-X antibody; neuronal migration protein doublecortin antibody; neuronal migration protein doublecortin antibody; SCLH antibody; X-linked (doublecortin) antibody; XLIS antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human DCX (rh DCX; O43602-2; Ala45-Val150). DCX specific IgG was purified by Human DCX affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human DCX. The detection limit for Human DCX is approximately 0.00245 ng/well.
IHC-P: 0.1-2 μg/mL
IP: 1-4 μg/mg of lysate
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Validations
DCX Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human DCX in human brain with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
DCX Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
DCX Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Background
DCX (doublecortin, N-GST chimera)contains 2 doublecortin domains and belongs to the doublecortin family. It is highly expressed in neuronal cells of fetal brain, but not expressed in other fetal tissues. In the adult, it is highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas. DCX is a microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. It may act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. DCX may in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. It may be part with LIS-1 of a overlapping, but distinct, signaling pathways that promote neuronal migration. Defects in DCX are the cause of lissencephaly X-linked type 1 and subcortical band heterotopia X-linked.
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References
- Des Portes V, et al. (1998) A novel CNS gene required for neuronal migration and involved in X-linked subcortical laminar heterotopia and lissencephaly syndrome. Cell. 92:51-61.
- Gleeson J G, et al. (998) Doublecortin, a brain-specific gene mutated in human X-linked lissencephaly and double cortex syndrome, encodes a putative signaling protein. Cell. 92:63-72.
- Ross M T, et al. (2005) The DNA sequence of the human X chromosome. Nature. 434:325-37.
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