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Product Name
Anti-DCX antibody
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Description
Mouse monoclonal to DCX
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Tested applications
FCM, IF, ICC/IF
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Species reactivity
Human DCX
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Alternative names
Dbct antibody; DC antibody; DBCN antibody; LISX antibody; SCLH antibody; XLIS antibody; DBCN antibody; Dbct antibody; DC antibody; doublecortex antibody; doublecortex antibody; doubling antibody; doubling antibody; lissencephalin-X antibody; lissencephalin-X antibody; lissencephaly antibody; LISX antibody; lis-X antibody; lis-X antibody; neuronal migration protein doublecortin antibody; neuronal migration protein doublecortin antibody; SCLH antibody; X-linked (doublecortin) antibody; XLIS antibody
- Immunogen
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Isotype
Mouse IgG2a
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human DCX (rh DCX; O43602-2; Ala45-Val150). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
FCM: 0.5-2 μg/Test
ICC/IF: 10-25 μg/mL
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Validations
DCX Antibody, Mouse MAb, Immunofluorescence
Immunofluorescence staining of Human DCX in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human DCX monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
DCX Antibody, Mouse MAb
Flow cytometric analysis of Human DCX expression on HepG2 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human DCX, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
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Background
DCX (doublecortin, N-GST chimera)contains 2 doublecortin domains and belongs to the doublecortin family. It is highly expressed in neuronal cells of fetal brain, but not expressed in other fetal tissues. In the adult, it is highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas. DCX is a microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. It may act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. DCX may in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. It may be part with LIS-1 of a overlapping, but distinct, signaling pathways that promote neuronal migration. Defects in DCX are the cause of lissencephaly X-linked type 1 and subcortical band heterotopia X-linked.
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References
- Des Portes V, et al. (1998) A novel CNS gene required for neuronal migration and involved in X-linked subcortical laminar heterotopia and lissencephaly syndrome. Cell. 92:51-61.
- Gleeson J G, et al. (998) Doublecortin, a brain-specific gene mutated in human X-linked lissencephaly and double cortex syndrome, encodes a putative signaling protein. Cell. 92:63-72.
- Ross M T, et al. (2005) The DNA sequence of the human X chromosome. Nature. 434:325-37.
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