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Product Name
Anti-Cytochrome C Rabbit antibody
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Description
Cytochrome C Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
CYC; HCS; THC4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human Cytochrome C
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
ICC/IF: 1/20-1/50
IP: 1/20
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Validations
Western blot detection of Cytochrome C in K562,Rat Brain,C6,3T3,Hela cell lysates using Cytochrome C Rabbit pAb(1:1000 diluted).Predicted band size:12kDa.Observed band size:14kDa.
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Background
Swiss-Prot Acc.P99999.Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.SitesFeature keyPosition(s)Description
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"