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Anti-Cxcr3 antibody

Cat.#: 175353

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-Cxcr3 antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to Cxcr3
  • Tested applications
    WB, FC
  • Species reactivity
    Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Guinea Pig
  • Alternative names
    Cmka antibody; Cxcr antibody; Cd183 antibody; Cmkar3 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was klh conjugated synthetic peptide derived from mouse cxcr3 301-367/367
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • Storage instructions
    Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
  • Applications

    WB:1:500-2000

    FC:0.2μg /test

  • Validations

    Fig1: Sample:; liver(mouse) Lysate at 40 ug; Primary: Anti-CXCR3 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 41kD; Observed band size: 51kD

    Fig1: Sample:; liver(mouse) Lysate at 40 ug; Primary: Anti-CXCR3 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 41kD; Observed band size: 51kD

    Fig2: Sample:; heart(mouse) Lysate at 40 ug; Primary: Anti-CXCR3 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 41kD; Observed band size: 51kD

    Fig2: Sample:; heart(mouse) Lysate at 40 ug; Primary: Anti-CXCR3 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 41kD; Observed band size: 51kD

    Fig3: Blank control: HCCLM3(blue); Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 5ul in 100 uL1X PBS containing 0.5% BSA(green).

    Fig3: Blank control: HCCLM3(blue); Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 5ul in 100 uL1X PBS containing 0.5% BSA(green).

    Fig4: Blank control: U937.; Primary Antibody (green line): Rabbit Anti-CXCR3 antibody ; Dilution: 1μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody : Goat anti-rabbit IgG-PE; Dilution: 1μg /test.; Protocol; The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

    Fig4: Blank control: U937.; Primary Antibody (green line): Rabbit Anti-CXCR3 antibody ; Dilution: 1μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody : Goat anti-rabbit IgG-PE; Dilution: 1μg /test.; Protocol; The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

  • Background
    O88410

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"