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Product Name
Anti-Clec5a antibody
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Description
Rabbit polyclonal antibody to Clec5a
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Tested applications
WB, IHC-P
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Species reactivity
Human, Mouse, Rat, Pig, Cow, Horse, Rabbit
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Alternative names
Ly10 antibody; MDL- antibody; Mdl1 antibody; Clecs antibody; Ly100 antibody; MDL-1 antibody; Clecsf5 antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was klh conjugated synthetic peptide derived from mouse clec5a 101-190/190
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Clonality
Polyclonal
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Formulation
Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
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Storage instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
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Applications
WB:1:500-2000
IHC-P:1:400-800
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Validations
Fig1: Cell: U937.; Concentration: 1:100.; Incubation: 40 minutes, room temperature.; Host/Blank: U937 cells.; Flow cytometric analysis of Rabbit Anti-CLEC5A antibody (green) compared with control in the absence of primary antibody (blue) followed by U937 cells.; secondary antibody: Goat Anti-rabbit IgG/FITC antibody (bs-0295G-FITC)
Fig2: Sample: Spleen (Mouse) Lysate at 40 ug; Primary: Anti-CLEC5A at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 22 kD; Observed band size: 22 kD
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