-
Product Name
Anti-CITED1 antibody
- Documents
-
Description
Rabbit polyclonal antibody to CITED1
-
Tested applications
WB, IHC-P
-
Species reactivity
Human, Mouse, Rat
-
Alternative names
MSG1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antigen of this antibody was klh conjugated synthetic peptide derived from human cited1/abcc1 121-193/193
-
Clonality
Polyclonal
-
Formulation
Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
-
Storage instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
-
Applications
WB:1:500-2000
IHC-P:1:400-800
-
Validations
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (CITED1 ) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig2: Sample:; Testis (Mouse) Lysate at 40 ug; Primary: Anti-CITED1 at 1/2000 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 20 kD; Observed band size: 20 kD
- Background
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"