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Product Name
Anti-Chk1 Rabbit antibody
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Description
Chk1 Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF, IP
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human Chk1
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-1/2000
ICC/IF: 1/50-1/200
IP: 1/30
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Validations
Western blot analysis of Chk1 expression in C2C12,THP-1,3T3,Jurakt and Hela cell lysates using Chk1 antibody at 1/1000 dilution.Predicted band size:54KDa.Observed band size:54KDa.
Western blot analysis of Chk1 in (1) K562 cell lysate; (2) PC-12 cell lysate.
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Background
Swiss-Prot Acc.O14757.DNA damage induced protein phosphorylation; regulation of mitotic centrosome separation; regulation of S phase; peptidyl-threonine phosphorylation; DNA repair; chromatin-mediated maintenance of transcription; negative regulation of mitosis;
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"