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Product Name
Anti-CDKN2A/p16INK4a Rabbit antibody
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Description
CDKN2A/p16INK4a Rabbit polyclonal antibody
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Tested applications
WB, ICC/IF, FC
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Species reactivity
Human
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Alternative names
ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of human CDKN2A/p16INK4a
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
ICC/IF: 1/20-1/100
FC: 1/20
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Validations
Immunocytochemistry of CDKN2A/p16INK4a(green) in Hela cells using CDKN2A/p16INK4a Rabbit pAb at dilution 1/50, and DAPI(blue)
Western blot detection of CDKN2A/p16INK4a in 293 cell lysates using CDKN2A/p16INK4a Rabbit pAb(1:1000 diluted).Predicted band size:17KDa.Observed band size:17KDa.
Western blot detection of CDKN2A-p16INK4a in 3T3,Hela cell lysates using CDKN2A/p16INK4a Rabbit pAb(1:1000 diluted).Predicted band size:17KDa.Observed band size:17KDa.
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Background
Swiss-Prot Acc.P42771.Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"