-
Product Name
Anti-CD28 antibody
- Documents
-
Description
Rabbit polyclonal to CD28
-
Tested applications
ELISA, WB
-
Species reactivity
Mouse CD28
-
Alternative names
Cd28 antibody; CD28 antibody; CH29-189K17.3 antibody; MGC138290 antibody; Tp44 antibody; Tp44 antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
Produced in rabbits immunized with purified, recombinant Mouse CD28 (rM CD28; NP_031668.3; Met1-Lys149). CD28 specific IgG was purified by Mouse CD28 affinity chromatography.
-
Clonality
Polyclonal
-
Formulation
0.2 μm filtered solution in PBS
-
Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-30 μg/ml
ELISA: 0.1-0.2 μg/ml
This antibody can be used at 0.1-0.2 μg/ml with the appropriate secondary reagents to detect Mouse CD28. The detection limit for Mouse CD28 is approximately 0.00245 ng/well.
-
Validations
CD28 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
-
Background
CD28 (Cluster of Differentiation 28) is a disulphide-bonded glycoprotein belonging to the immunoglobulin (Ig) superfamily, and structurally consists of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. Mouse CD28 is constitutively expressed on the surface of all murine T cells and on developing thymocytes as disulfide-linked homodimers or as monomers. CD28 can binds the B7-1 and B7-2 ligand, and together perform important functions in the T and B cell response pathways. B7/CD28 family members, which can augment or antagonize T-cell receptor signaling, in the regulation of central and peripheral T-cell tolerance. CD28 is thus involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
-
References
- Keir ME, et al. (2005) The B7/CD28 costimulatory family in autoimmunity. Immunol Rev. 204: 128-43.
- Sansom DM, et al. (2006) The role of CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4) in regulatory T-cell biology. Immunol Rev. 212: 131-48.
- Bjrgo E, et al. (2010) Novel mechanism of signaling by CD28. Immunol Lett. 129(1): 1-6.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"