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Product Name
Anti-CD136/MST1R antibody
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Description
Rabbit polyclonal to CD136/MST1R
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Tested applications
ELISA, IHC-P
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Species reactivity
Human CD136 / MST1R
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Alternative names
RON antibody; PTK8 antibody; CD136 antibody; CDw136 antibody; Fv2 antibody; Ron antibody; STK antibody; Fv-2 antibody; PTK8 antibody; CD136 antibody; CDw136 antibody; Cd136 antibody; CD136 antibody; CDw136 antibody; CDw136 antibody; Fv2 antibody; Fv-2 antibody; Mst1r antibody; MST1R antibody; PTK8 antibody; PTK8 antibody; Ron antibody; RON antibody; STK antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human CD136 / MST1R (rh CD136 / MST1R; Q04912; Met1-Leu571). CD136 / MST1R specific IgG was purified by Human CD136 / MST1R affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/ml
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD136 / MST1R.
IHC-P: 5-20 μg/ml
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Validations
CD136 / MST1R Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human TNFRSF10D in human liver with mouse monoclonal antibody (10 µg/mL, formalin-fixed paraffin embedded sections).
CD136 / MST1R Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human TNFRSF10D in human kidney with mouse monoclonal antibody (10 µg/mL, formalin-fixed paraffin embedded sections).
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Background
The tyrosine kinase receptor, macrophage-stimulating 1 receptor (MST1R), a c-met-related tyrosine kinase, also known as the Ron receptor or CD136, controls cell survival and motility programs related to invasive growth. As tyrosine kinase receptor comprised of an extra-cellular domain, MST1R protein contains the ligand binding pocket and an intracellular region where the kinase domain is located. MST1R signaling may be involved in the regulation of macrophage and T-lymphocyte activation in vivo during injury. This assessment of gene expression indicates the importance of genetic factors in contributing to lung injury, and points to strategies for intervention in the progression of inflammatory diseases. It had been shown that MST1R/CD136 plays a critical role in Ni-induced lung injury in mice. The overexpression of MSP, MT-SP1, and MST1R was a strong independent indicator of both metastasis and death in human breast cancer patients and significantly increased the accuracy of an existing gene expression signature for poor prognosis. Stimulation of MST1R leads to its transphosphorylation and the ultimate activation of numerous intracellular signaling pathways, such as the classical mitogen-activated protein kinase pathway, the phosphotidylinositol (PI)3-kinase pathway, and the JNK pathway.
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References
- Ronsin C, et al. (1993) A novel putative receptor protein tyrosine kinase of the met family. Oncogene. 8: 1195-1202.
- McDowell SA, et al. (2002) The role of the receptor tyrosine kinase Ron in nickel-induced acute lung injury. Am J Respir Cell Mol Biol. 26(1): 99-104.
- Angeloni D, et al. (2003) C to A single nucleotide polymorphism in intron 18 of the human MST1R (RON) gene that maps at 3p21.3. Mol Cell Probes. 17(2-3): 55-7.
- Mallakin A, et al. (2006) Gene expression profiles of Mst1r-deficient mice during nickel-induced acute lung injury. Am J Respir Cell Mol Biol. 34(1): 15-27.
- Welm AL, et al. (2007) The macrophage-stimulating protein pathway promotes metastasis in a mouse model for breast cancer and predicts poor prognosis in humans. Proc Natl Acad Sci U S A. 104(18): 7570-5.
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