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Product Name
Anti-Calsequestrin-1 / CASQ1 antibody
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Description
Rabbit polyclonal to Calsequestrin-1 / CASQ1
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Tested applications
ELISA, IHC-P
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Species reactivity
Human Calsequestrin-1 / CASQ1
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Alternative names
calsequestrin 1 (fast-twitch antibody; skeletal muscle) antibody; CASQ1 antibody; RP11-536C5.6 antibody; CASQ antibody; PDIB1 antibody; calmitin antibody; calmitine antibody; calsequestrin antibody; skeletal muscle isoform antibody; calsequestrin-1 antibody; calsequestrin 1 antibody; Casq1 antibody; CSQ antibody; CSQ-1 antibody; CSQ1 antibody; sCSQ antibody; calsequestrin antibody; skeletal muscle isoform antibody; CASQ antibody; PDIB1 antibody; VMCQA antibody; CSQ antibody; CSQ1 antibody; sCSQ antibody; CSQ-1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human Calsequestrin-1 / CASQ1 (rh Calsequestrin-1 / CASQ1; P31415; Gln35-Asp396). Calsequestrin-1 / CASQ1 specific IgG was purified by Human Calsequestrin-1 / CASQ1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/ml
This antibody can be used at 0.1-0.2 μg/ml with the appropriate secondary reagents to detect Human Calsequestrin-1 / CASQ1.
IHC-P: 0.1-2 μg/mL
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Validations
Calsequestrin-1 / CASQ1 Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of human CASQ1 in human heart with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
Calsequestrin-1 / CASQ1 Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of human CASQ1 in human skeletal muscle with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Calsequestrin-1 is an isoform of calsequestrin. Calsequestrin is a calcium-binding protein of the sarcoplasmic reticulum. It helps hold calcium in the cisterna of the sarcoplasmic reticulum after a muscle contraction, even though the concentration of calcium in the sarcoplasmic reticulum is much higher than in the cytosol. Two forms of calsequestrin have been identified: Calsequestrin-2 and Calsequestrin-1. Calsequestrin-1 is found in fast skeletal muscle. The release of calsequestrin-bound calcium (through a calcium release channel) triggers muscle contraction. The active protein is not highly structured, more than 50% of it adopting a random coil conformation. When calcium binds there is a structural change whereby the alpha-helical content of the protein increases from 3 to 11%. Both forms of calsequestrin are phosphorylated by casein kinase 2, but the cardiac form is phosphorylated more rapidly and to a higher degree. Calsequestrin-1 is also secreted in the gut where it deprives bacteria of calcium ions.
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References
- Slupsky JR, et al. (1987) Characterization of cardiac calsequestrin. Biochemistry. 26(20): 6539-44.
- Cala SE, et al. (1991) Phosphorylation of cardiac and skeletal muscle calsequestrin isoforms by casein kinase II. Demonstration of a cluster of unique rapidly phosphorylated sites in cardiac calsequestrin. J Biol Chem. 266(1):391-8.
- Wang S, et al. (1998) Crystal structure of calsequestrin from rabbit skeletal muscle sarcoplasmic reticulum. Nat Struct Biol. 5(6):476-83.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"