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Product Name
Anti-CALML5 antibody
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Description
Rabbit polyclonal to CALML5
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Tested applications
ELISA, IHC-P
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Species reactivity
Human CALML5
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Alternative names
CALML5 antibody; CLSP antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human CALML5 (rh CALML5; AAH39172.1; Met 1-Glu 146). CALML5 specific IgG was purified by Human CALML5 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CALML5. The detection limit for Human CALML5 is approximately 0.00245 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
CALML5 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human CALML5 in human mammary adenocarcinoma with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.
CALML5 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human CALML5 in human skin (from donor 1) with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
CALML5 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human CALML5 in human skin (from donor 2) with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.
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Background
Calmodulin-like protein 5, also known as Calmodulin-like skin protein, CALML5 and CLSP, is a protein which contains four EF-hand domains. CALML5 / CLSP is particularly abundant in the epidermis where its expression is directly related to keratinocyte differentiation.The expression is very low in lung. CALML5 / CLSP binds calcium. It may be involved in terminal differentiation of keratinocytes. Coxsackievirus and adenovirus receptor (CAR) is a member of the immunoglobulin (Ig) superfamily and a component of epithelial tight junction. CAR functions as a primary receptor for coxsackievirus B and adenovirus (Ad) infection. CALML5 / CLSP is closely related to CAR. The structure and dynamics of human calmodulin-like skin protein CALML5 / CLSP have been characterized by NMR spectroscopy. The mobility of CALML5 / CLSP has been found to be different for the N-terminal and C-terminal domains. The N-terminal domain is characterized by four stable helices, which experience large fluctuations. This is shown to be due to mutations in the hydrophobic core. The overall N-terminal domain behavior is similar both in the full-length protein and in the isolated domain.
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References
- Mehul B., et al., 2000, J. Biol. Chem. 275:12841-12847.
- Babini E., et al., 2006, Structure 14:1029-1038.
- Kawabata,K. et al., 2007, Gene Ther. 14 (16):1199-207.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"