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Product Name
CALML5 antibody
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Description
CALML5 Rabbit Polyclonal antibody. Positive IP detected in A431 cells. Positive WB detected in A431 cells. Positive IHC detected in human skin cancer tissue. Observed molecular weight by Western-blot: 16 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
CALML5 antibody; calmodulin like 5 antibody; Calmodulin like protein 5 antibody; Calmodulin like skin protein antibody; CLSP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CALML5 recombinant protein (Accession Number: NM_017422). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:108828(CALML5 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:108828(CALML5 antibody) at dilution of 1:500 (under 10x lens)
Immunohistochemical of paraffin-embedded human skin cancer using Catalog No:108828(CALML5 antibody) at dilution of 1:500 (under 40x lens)
IP Result of anti-CALML5 (IP:Catalog No:108828, 3ug; Detection:Catalog No:108828 1:300) with A431 cells lysate 1600ug.
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Background
CALML5, also known as calmodulin-like skin protein (CLSP), a recently discovered skin-specific calcium-binding protein, is closely related to keratinocyte differentiation. Abundant expression is detected only in reconstructed epidermis and is restricted to differentiating keratinocytes. In addition, it can associate with transglutaminase 3, shown to be a key enzyme in the terminal differentiation of keratinocytes.
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References
- AbdulMajeed AA, Dalley AJ, Farah CS. Loss of ELF3 immunoexpression is useful for detecting oral squamous cell carcinoma but not for distinguishing between grades of epithelial dysplasia. Annals of diagnostic pathology. 17(4):331-40. 2013.
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