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Product Name
Anti-Biotinidase / biotinase / BTD antibody
- Documents
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Description
Rabbit polyclonal to Biotinidase / biotinase / BTD
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Tested applications
ELISA, WB, IHC-P
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Species reactivity
Human Biotinidase / biotinase / BTD
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Alternative names
Biotinidase antibody; Btd antibody; biotinase antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human Biotinidase / biotinase / BTD (rh Biotinidase / biotinase / BTD; P43251; Met1-Asp543). Biotinidase / biotinase / BTD specific IgG was purified by Human Biotinidase / biotinase / BTD affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human Biotinidase / biotinase / BTD. The detection limit for Human Biotinidase / biotinase / BTD is < 0.039 ng/well.
IHC-P: 0.1-2 μg/ml
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Validations
Biotinidase / biotinase / BTD Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
Biotinidase / biotinase / BTD Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Biotinidase / biotinase / BTD Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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Background
Biotinidase, also known as biotinase and BTD, is a ubiquitous mammalian cell enzyme which expressed at high levels in the liver, serum, and kidney. Its primary function is to cleave biotin from biocytin, preserving the pool of biotin for use as a cofactor for biotin dependent enzymes, namely the 4 human carboxylases. Biotinidase also recycles biotin from enzymes in the body that use it as a helper component in order to function. These enzymes, known ascarboxylases, are important in the processing of fats, carbohydrates, and proteins. Biotin is attached to these carboxylase enzymes through an amino acid (the building material of proteins) called lysine, forming a complex calledbiocytin.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"