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Product Name
Anti-Biotinidase / biotinase / BTD antibody
- Documents
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Description
Rabbit monoclonal to Biotinidase / biotinase / BTD
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Tested applications
ELISA, IHC-P
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Species reactivity
Human Biotinidase / biotinase / BTD
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Alternative names
Biotinidase antibody; Btd antibody; biotinase antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Biotinidase / biotinase / BTD (rh Biotinidase / biotinase / BTD; P43251; Met1-Asp543).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL μg/ml with the appropriate secondary reagents to detect Human Biotinidase / biotinase / BTD.
IHC-P: 1-10 μg/mL
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Validations
Biotinidase / biotinase / BTD Antibody, Rabbit MAb, Immunohistochemistry
Immunochemical staining of human BTD in human liver with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
Biotinidase / biotinase / BTD Antibody, Rabbit MAb, Immunohistochemistry
Immunochemical staining of human BTD in human kidney with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Biotinidase, also known as biotinase and BTD, is a ubiquitous mammalian cell enzyme which expressed at high levels in the liver, serum, and kidney. Its primary function is to cleave biotin from biocytin, preserving the pool of biotin for use as a cofactor for biotin dependent enzymes, namely the 4 human carboxylases. Biotinidase also recycles biotin from enzymes in the body that use it as a helper component in order to function. These enzymes, known ascarboxylases, are important in the processing of fats, carbohydrates, and proteins. Biotin is attached to these carboxylase enzymes through an amino acid (the building material of proteins) called lysine, forming a complex calledbiocytin.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"