Anti-Biotinidase / biotinase / BTD antibody

Cat.#: 101569

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Product Information

  • Product Name
    Anti-Biotinidase / biotinase / BTD antibody
  • Documents
  • Description
    Rabbit monoclonal to Biotinidase / biotinase / BTD
  • Tested applications
    ELISA, IHC-P
  • Species reactivity
    Human Biotinidase / biotinase / BTD
  • Alternative names
    Biotinidase antibody; Btd antibody; biotinase antibody
  • Immunogen
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained from a rabbit immunized with purified, recombinant Human Biotinidase / biotinase / BTD (rh Biotinidase / biotinase / BTD; P43251; Met1-Asp543).
  • Clonality
    Monoclonal
  • Formulation
    0.2 μm filtered solution in PBS
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    ELISA: 0.1-0.2 μg/mL

    This antibody can be used at 0.1-0.2 μg/mL μg/ml with the appropriate secondary reagents to detect Human Biotinidase / biotinase / BTD.

    IHC-P: 1-10 μg/mL

  • Validations

    Biotinidase / biotinase / BTD Antibody, Rabbit MAb, Immunohistochemistry

    Biotinidase / biotinase / BTD Antibody, Rabbit MAb, Immunohistochemistry

    Immunochemical staining of human BTD in human liver with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).

    Biotinidase / biotinase / BTD Antibody, Rabbit MAb, Immunohistochemistry

    Biotinidase / biotinase / BTD Antibody, Rabbit MAb, Immunohistochemistry

    Immunochemical staining of human BTD in human kidney with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).

  • Background
    Biotinidase, also known as biotinase and BTD, is a ubiquitous mammalian cell enzyme which expressed at high levels in the liver, serum, and kidney. Its primary function is to cleave biotin from biocytin, preserving the pool of biotin for use as a cofactor for biotin dependent enzymes, namely the 4 human carboxylases. Biotinidase also recycles biotin from enzymes in the body that use it as a helper component in order to function. These enzymes, known ascarboxylases, are important in the processing of fats, carbohydrates, and proteins. Biotin is attached to these carboxylase enzymes through an amino acid (the building material of proteins) called lysine, forming a complex calledbiocytin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"