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Product Name
Anti-Bax Rabbit antibody
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Description
Bax Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, IP, FC
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Species reactivity
Human, Mouse, Rat, Hamster
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human Bax
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000-1/2000
IHC: 1/50-1/200
IP: 1/50
FC: 1/50
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Validations
Western blot analysis of Bax in (1) HeLa cell lysate;(2) RAW264.7 Whole Cell Lysate.
Western blot analysis of extracts from THP-1 and Hela cells using Bax Rabbit pAb at 1:1000 dilution. Predicted band size: 21kDa. Observed band size: 21kDa.
Immunohistochemical analysis of paraffin-embedded human adenocarcinoma of colon, using Bax Antibody.
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Background
Swiss-Prot Acc.Q07812.Bax is a key component for cellular induced apoptosis through mitochondrial stress. Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane. Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"