Product Name

Anti-B7-H4/B7S1/B7x antibody

Documents

Description

Rabbit polyclonal to B7-H4/B7S1/B7x

Tested applications

ELISA, IHC-P

Species reactivity

Human B7-H4 / B7S1 / B7x

Alternative names

immune costimulatory protein B7-H4 antibody; t cell costimulatory molecule B7x antibody; B7h4 antibody; B7s1 antibody; B7x antibody; BC032925 antibody; V-set domain containing T-cell activation inhibitor 1 antibody

Immunogen

Human B7-H4/B7S1/B7x (His Tag) recombinant protein

Isotype

Rabbit IgG

Preparation

Produced in rabbits immunized with purified, recombinant Human B7-H4 / B7S1 / B7x (rh B7-H4 / B7S1 / B7x; Q7Z7D3; Phe29-Ala258). B7-H4 / B7S1 / B7x specific IgG was purified by Human B7-H4 / B7S1 / B7x affinity chromatography.

Clonality

Polyclonal

Formulation

0.2 μm filtered solution in PBS

Storage instructions

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Applications

ELISA:0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human B7-H4 / B7S1 / B7x. The detection limit for Human B7-H4 / B7S1 / B7x is < 0.039 ng/well.

IHC-P: 0.1-1 μg/ml

Validations

B7-H4 / B7S1 / B7x Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry

Background

V-set domain-containing T-cell activation inhibitor 1, also known as B7X, B7H4, B7S1, and VTCN1, is a single-pass type? membrane protein belonging to the B7 family of costimulatory proteins. These proteins are expressed on the surface of antigen-presenting cells and interact with ligands on T lymphocytes. They provide costimulatory signals that regulate T cell responses. A soluble form of B7H4 has also been detected. B7X / VTCN1 / B7H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, B7X / VTCN1 / B7H4 plays an important role, together with regulatory T-cells(Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. B7X / VTCN1 / B7H4 is also involved in promoting epithelial cell transformation. This membrane protein can be up-regulated by IL6 / interleukin-6 and IL10 / interleukin-10 and inhibited by CSF2 / GM-CSF and IL4 / interleukin-4 on antigen-presenting cells.

References

Zang X, et al. (2003) B7x: a widely expressed B7 family member that inhibits T cell activation. Proc Natl Acad Sci U S A. 100(18): 10388-92.
Suh WK, et al. (2006) Generation and characterization of B7-H4/B7S1/B7x-deficient mice. Mol Cell Biol. 26(17): 6403-11.
Zang X, et al. (2007) B7-H3 and B7x are highly expressed in human prostate cancer and associated with disease spread and poor outcome. Proc Natl Acad Sci U S A. 104(49):19458-63.

Anti-B7-H4/B7S1/B7x antibody

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