-
Product Name
Anti-B2M/beta-2 microglobulin antibody
- Documents
-
Description
Rabbit polyclonal to B2M/beta-2 microglobulin
-
Tested applications
ELISA, WB, IP
-
Species reactivity
Cynomolgus B2M / beta-2 microglobulin
-
Alternative names
B2M antibody; B2m antibody; beta-2 microglobulin antibody; beta-2 microglobulin antibody; beta2m antibody; beta2-m antibody; beta-2-microglobin antibody; beta-2-microglobin antibody; CDABP0092 antibody; Ly-m11 antibody; RP23-34E24.5 antibody; beta chain of MHC class I molecules antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
Produced in rabbits immunized with purified, recombinant Cynomolgus B2M / beta-2 microglobulin (Q6V7J5; Met1-Met119). B2M / beta-2 microglobulin specific IgG was purified by Cynomolgus B2M / beta-2 microglobulin affinity chromatography.
-
Clonality
Polyclonal
-
Formulation
0.2 μm filtered solution in PBS
-
Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 2-10 μg/ml
ELISA: 0.1-0.2 μg/ml
This antibody can be used at 0.1-0.2 μg/ml with the appropriate secondary reagents to detect Cynomolgus B2M / beta-2 microglobulin. The detection limit for Cynomolgus B2M / beta-2 microglobulin is approximately < 0.039 ng/well.
IP: 1-4 μg/mg of lysate
-
Validations
B2M / beta-2 microglobulin Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
B2M / beta-2 microglobulin Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
Immunochemical staining of human CLSTN1 in human brain with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
-
Background
B2M, also known as β2-Microglobulin or CDABP0092, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells). The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M(β2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M(β2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens.B2M(β2- Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to theα3 chain. Unlikeα3, β2 has no transmembrane region. Directly above β2 lies the α1 chain, which itself is lateral to the α2. In the absence of B2M(β2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M(β2 microglobulin) can indicate non-progression of HIV.
-
References
- Poulik MD, et al. (1979) Beta 2-Microglobulin: methods and clinical applications. CRC Ctit Rev Clin Lab Sci. 10(3): 225-45.
- Poulik MD, et al. (1975) Beta2-Microglobulins. Contemp Top Mol Immunol. 4: 157-204.
- Berggard I. (1976) Beta2-Microglobulins: isolation, properties, and distribution. Fed Proc. 35(5): 1167-70.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"