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Product Name
Beta-2-Microglobulin Polyclonal antibody
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Description
Beta-2-microglobulin Rabbit Polyclonal antibody. Positive IHC detected in human oesophagus cancer tissue, human stomach cancer tissue. Positive IF detected in A431 cells. Positive FC detected in HeLa cells. Positive WB detected in HeLa cells, A431 cells, human heart tissue, human stomach tissue, mouse lung tissue, Raji cells. Observed molecular weight by Western-blot: 12 kDa
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Tested applications
ELISA, WB, FC, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
B2M antibody; Beta-2-microglobulin antibody; HDCMA22P antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Beta-2-microglobulin recombinant protein (Accession Number: NM_004048). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB : 1:1000-1:8000
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
IHC : 1:2000-1:8000
IF/ICC : 1:375-1:1500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:117131(B2M antibody) at dilution of 1:1000
1X10^6 HeLa cells were stained with 0.2ug B2M antibody (Catalog No:117131, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Immunohistochemistry of paraffin-embedded human oesophagus cancer tissue slide using Catalog No:117131(B2M Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human oesophagus cancer tissue slide using Catalog No:117131(B2M Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed A431 cells using Catalog No:117131(B2M Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.
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References
- Ma Y, Shen B, Zhang X. Heritable multiplex genetic engineering in rats using CRISPR/Cas9. PloS one. 9(3):e89413. 2014.
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