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Product Name
Anti-ATF4 Rabbit antibody
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Description
ATF4 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP, FC
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human ATF4
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500-1/1000
IHC: 1/50-1/200
ICC: 1/100-1/500
IP: 1/50
FC: 1/50
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Validations
Immunohistochemical analysis of paraffin-embedded mouse kidney, using ATF4 Antibody.
Western blot analysis of ATF4 expression in (1) HeLa cell lysate; (2) 3T3 cell lysate.
Immunofluorescent analysis of 293 cells, using ATF4 Antibody.
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Background
Swiss-Prot Acc.P18848.ATF4, an activating transcription factor/cAMP-response element-binding protein family member, functions in the PERK and eIF2α ER stress responsive pathway. ER stress represses the translation of the majority of mRNAs, but selectively stimulates the translation of certain mRNAs including that of ATF-4. Induced expression of ATF-4 increases the expression of genes critical for the recovery from ER stress.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"