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Product Name
Anti-APG5L/ATG5 Rabbit pA
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Description
APG5L/ATG5 Rabbit pA
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Tested applications
WB, IHC-P, ICC/IF, IP
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human ATG5
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000-1/2000
IHC: 1/50-1/200
ICC/IF: 1/50-1/200
IP: 1/50
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Validations
Immunofluorescent analysis of Raji cells, using ATG5 Antibody.
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using ATG5 Antibody.
Western blot analysis of extracts from U87MG and Raji cells using ATG5 Rabbit pAb at 1:1000 dilution. Predicted band size: 55kDa. Observed band size: 55kDa.
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Background
Swiss-Prot Acc.Q9H1Y0.Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"