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Product Name
Anti- antibody
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Description
Rabbit polyclonal antibody to
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Tested applications
IF, IHC-P
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Species reactivity
Arabidopsis thaliana
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Alternative names
ECH antibody; ECHIDNA antibody; T31J12.5 antibody; T31J12_5 antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was synthetic peptide within arath ech aa 1-25.
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Clonality
Polyclonal
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Formulation
Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
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Applications
IF:1:50-1:200
IHC-P:1:50-1:200
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Validations
Fig1:; IF staining of ECH in Arabidopsis thaliana leaf tissue (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig2:; Immunohistochemical analysis of paraffin-embedded Arabidopsis thaliana leaf tissue using anti-ECH antibody. Counter stained with hematoxylin.
- Background
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References
- Gendre D et al. Conserved Arabidopsis ECHIDNA protein mediates trans-Golgi-network trafficking and cell elongation. Proc. Natl. Acad. Sci. U.S.A. 108:8048-8053 (2011).
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