Anti-Alpha Skeletal Muscle Actin (2B10) Mouse antibody

Cat.#: 169058

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Product Information

  • Product Name
    Anti-Alpha Skeletal Muscle Actin (2B10) Mouse antibody
  • Documents
  • Description
    Alpha Skeletal Muscle Actin (2B10) Mouse monoclonal antibody
  • Tested applications
    WB, IHC-P, ICC/IF, IP
  • Species reactivity
    Human, Mouse, Rat
  • Isotype
    Mouse IgG
  • Preparation
    Antigen: Synthetic Peptide of α skeletal muscle actin
  • Clonality
    Monoclonal
  • Formulation
    PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500-10000

    IP:1/200

    IF: 1/200

    IHC: 1/50-300

  • Validations

    Western blot detection of Alpha Skeletal Muscle Actin in T47D,3T3,COS7,C6 and Hela cell lysates using Alpha Skeletal Muscle Actin mouse mAb (1:10000 diluted).Predicted band size:42KDa.Observed band size:42KDa.

    Western blot detection of Alpha Skeletal Muscle Actin in T47D,3T3,COS7,C6 and Hela cell lysates using Alpha Skeletal Muscle Actin mouse mAb (1:10000 diluted).Predicted band size:42KDa.Observed band size:42KDa.

    Western blot analysis of 1) Hela, 2) Mouse Brain tissue, 3) Rat Brain tissue with  diluted at 1:20,000.

    Western blot analysis of 1) Hela, 2) Mouse Brain tissue, 3) Rat Brain tissue with diluted at 1:20,000.

    Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1,α skeletal muscle actin Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

    Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1,α skeletal muscle actin Monoclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

  • Background
    Swiss-Prot Acc.P68133.Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.MiscellaneousIn vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"