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Product Name
Anti-AGO2 / Argonaute 2 antibody
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Description
Rabbit polyclonal to AGO2 / Argonaute 2
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Tested applications
WB, IHC-P, IP
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Species reactivity
Human AGO2 / Argonaute 2
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Alternative names
Q10 antibody; EIF2C2 antibody; Eif2c2 antibody; Gerp95 antibody; Gm10365 antibody; AI225898 antibody; AL022874 antibody; AW546247 antibody; mKIAA4215 antibody; 1110029L17Rik antibody; 2310051F07Rik antibody; ENSMUSG00000072493 antibody; Ago2 antibody; AGO2 antibody; argonaute 2 antibody; argonaute 2 antibody; argonaute2 antibody; argonaute2 antibody; eIF2C 2 antibody; eIF2C 2 antibody; eIF-2C 2 antibody; eIF-2C 2 antibody; Eif2c2 antibody; EIF2C2 antibody; Gerp95 antibody; Gm10365 antibody; hAgo2 antibody; KIAA4215 antibody; mAgo2ENSMUSG00000072493 antibody; MGC3183 antibody; mKIAA4215 antibody; protein slicer antibody; protein slicer antibody; Q10 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human AGO2 / Argonaute 2 (rh AGO2 / Argonaute 2; NP_036286.2; Met1-Ala859). AGO2 / Argonaute 2 specific IgG was purified by Human AGO2 / Argonaute 2 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-30 μg/ml
IHC-P: 0.1-2 μg/mL
IP: 1-4 μg/mg of lysate
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Validations
AGO2 / Argonaute 2 Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of human AGO2 in human stomach with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
AGO2 / Argonaute 2 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
AGO2 / Argonaute 2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
AGO2 / Argonaute 2 Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of human AGO2 in human brain with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 regulates dFMR1 expression, and the relationship between dFMR1 and AGO2 was defined by their physical interaction and co-regulation of downstream targets. AGO2 and dFMR1 are also connected through a regulatory relationship. AGO2 is a regulator of dFMR1 expression and have clarified an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines.
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References
- O'Carroll D, et al. (2007) A Slicer-independent role for Argonaute 2 in hematopoiesis and the microRNA pathway. Genes Dev. 21(16): 1999-2004.
- Pepper AS, et al. (2009) Argonaute2 suppresses Drosophila fragile X expression preventing neurogenesis and oogenesis defects. PLoS One. 4(10): e7618.
- Lima WF, et al. (2009) Binding and cleavage specificities of human Argonaute2. J Biol Chem. 284(38): 26017-28.
- Adams BD, et al. (2009) Argonaute-2 expression is regulated by epidermal growth factor receptor and mitogen-activated protein kinase signaling and correlates with a transformed phenotype in breast cancer cells. Endocrinology. 150(1): 14-23.
- Salvatore V, et al. (2010) Bacterial expression of mouse argonaute 2 for functional and mutational studies. Int J Mol Sci. 11(2): 745-53.
- Wilson JA, et al. (2011) Human Ago2 is required for efficient microRNA 122 regulation of hepatitis C virus RNA accumulation and translation. J Virol. 85(5): 2342-50.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"