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Product Name
Anti-ADGRF1 antibody
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Description
Rabbit polyclonal antibody to ADGRF1
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Tested applications
WB, FC
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Species reactivity
Human, Mouse
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Alternative names
PGR19 antibody; GPR110 antibody; KPG_012 antibody; hGPCR36 antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was klh conjugated synthetic peptide derived from human g protein coupled receptor 110 51-150/910
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Clonality
Polyclonal
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Formulation
Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
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Storage instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
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Applications
WB:1:500-2000
FC:1μg/Test
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Validations
Fig1: Blank control(blue): Mouse Kidney Cells(fixed with 2% paraformaldehyde (10 min)).; Primary Antibody:Rabbit Anti- GPR110 antibody , Dilution: 1ug in 100 uL 1X PBS containing 0.5% BSA;; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Fig2: Sample: A431 (human)Cell Lysate at 40 ug; Primary: Anti-GPR110 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 99 kD; Observed band size: 109 kD
Fig3: Sample: A549 (human)Cell Lysate at 40 ug; Primary: Anti-GPR110 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 99 kD; Observed band size: 109 kD
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"