-
Product Name
Annexin IV antibody
- Documents
-
Description
Annexin IV Mouse Monoclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human placenta tissue, human stomach cancer tissue. Positive WB detected in human heart tissue, COLO 320 cells. Observed molecular weight by Western-blot: 32 kDa
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
Human; other species not tested.
-
Alternative names
35-beta calcimedin antibody; Annexin A4 antibody; Annexin IV antibody; Annexin-4 antibody; Chromobindin-4 antibody; Endonexin I antibody; LipOCortin IV antibody
-
Isotype
Mouse IgG2c
-
Preparation
This antibody was obtained by immunization of Annexin IV recombinant protein (Accession Number: XM_047444081). Purification method: Protein A purified.
-
Clonality
Monoclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:50-1:500
IF: 1:50-1:500
-
Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:107057(Annexin IV Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human placenta tissue slide using Catalog No:107057(Annexin IV Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human placenta tissue slide using Catalog No:107057(Annexin IV Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:107057(Annexin IV Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
-
Background
Annexin IV /placental anticoagulant protein II (ANX4) is a member of the annexin or lipocortin family of calcium-dependent phospholipid binding proteins, and is located at 2p13. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 shares 45 to 59% identity with other members and possibly interacts with ATP. ANX4 has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"