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Product Name
ANKRD2 antibody
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Description
ANKRD2 Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue, human brain tissue, human lung tissue, human ovary tissue, human placenta tissue, human renal cell carcinoma tissue, human skeletal muscle tissue, human skin tissue, human testis tissue. Positive IP detected in mouse skeletal muscle tissue. Positive WB detected in human heart tissue, human skeletal muscle tissue, mouse skeletal muscle tissue. Observed molecular weight by Western-blot: 42kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ANKRD2 antibody; ARPP antibody; hArpp antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ANKRD2 recombinant protein (Accession Number: NM_001346797). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:108071(ANKRD2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human kidney using Catalog No:108071(ANKRD2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:108071(ANKRD2 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-ANKRD2 (IP:Catalog No:108071, 4ug; Detection:Catalog No:108071 1:500) with mouse skeletal muscle tissue lysate 3600ug.
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Background
ANKRD2 belongs to the conserved muscle ankyrin repeat protein (MARP) family. Expression of MARPs is induced in response to physiologic stress, injury, and hypertrophy. Together with Ankrd1/CARP and DARP, ANKRD2 are members of the MARP mechanosensing proteins that form a complex with titin (N2A)/calpain 3 protease/myopalladin. Ankrd2 is thought to have dual, structural and signaling roles, and could link the elastic I-band region as a stress sensor for transcriptional control in the nucleus
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References
- Cenni V, Bavelloni A, Beretti F. Ankrd2/ARPP is a novel Akt2 specific substrate and regulates myogenic differentiation upon cellular exposure to H(2)O(2). Molecular biology of the cell. 22(16):2946-56. 2011.
- Roberts MD, Childs TE, Brown JD, Davis JW, Booth FW. Early depression of Ankrd2 and Csrp3 mRNAs in the polyribosomal and whole tissue fractions in skeletal muscle with decreased voluntary running. Journal of applied physiology (Bethesda, Md. : 1985). 112(8):1291-9. 2012.
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