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Product Name
Amphiphysin antibody
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Description
Amphiphysin Rabbit Polyclonal antibody. Positive IHC detected in human pancreas cancer tissue. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, mouse lung tissue. Observed molecular weight by Western-blot: 115-125 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AMPH antibody; AMPH1 antibody; Amphiphysin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Amphiphysin recombinant protein (Accession Number: NM_001635). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108004(AMPH antibody) at dilution of 1:1500
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:108004(AMPH antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:108004(AMPH antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-AMPH (IP:Catalog No:108004, 4ug; Detection:Catalog No:108004 1:1500) with mouse brain tissue lysate 4000ug.
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Background
Amphiphysin (AMPH), a synaptic vesicle-associated protein that is highly concentrated in nerve terminal, is an autoantigen of Stiff-Man syndrome with breast cancer. Amphiphysin contains a N-terminal BAR (Bin1/Amphiphysin/Rv167) domain followed by an endocytosis domain, and a C-terminal SH3 domain. Amphiphysin has been proposed to function as a linker between the clathrin coat and dynamin in the endocytosis of synaptic vesicles. It may also participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. (PMID: 8552632; 8245793; 10559861)
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References
- Karner CM, Das A, Ma Z. Canonical Wnt9b signaling balances progenitor cell expansion and differentiation during kidney development. Development (Cambridge, England). 138(7):1247-57. 2011.
- Das A, Tanigawa S, Karner CM. Stromal-epithelial crosstalk regulates kidney progenitor cell differentiation. Nature cell biology. 15(9):1035-44. 2013.
- Hilliard SA, Yao X, El-Dahr SS. Mdm2 is required for maintenance of the nephrogenic niche. Developmental biology. 387(1):1-14. 2014.
- Hum S, Rymer C, Schaefer C, Bushnell D, Sims-Lucas S. Ablation of the renal stroma defines its critical role in nephron progenitor and vasculature patterning. PloS one. 9(2):e88400. 2014.
- Rymer C, Paredes J, Halt K. Renal blood flow and oxygenation drive nephron progenitor differentiation. American journal of physiology. Renal physiology. 307(3):F337-45. 2014.
- Boivin FJ, Sarin S, Lim J. Stromally expressed β-catenin modulates Wnt9b signaling in the ureteric epithelium. PloS one. 10(3):e0120347. 2015.
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