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Product Name
ALR antibody
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Description
ALR Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue, human liver tissue, human testis tissue. Positive WB detected in A375 cells, A431 cells, mouse liver tissue, rat liver tissue. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 23-25 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ALR antibody; ERV1 antibody; GFER antibody; Hepatopoietin antibody; HERV1 antibody; HPO antibody; HPO1 antibody; HPO2 antibody; HSS antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ALR recombinant protein (Accession Number: BC028348). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:107883(GFER antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:107883(GFER antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-GFER (IP:Catalog No:107883, 3ug; Detection:Catalog No:107883 1:1000) with mouse liver tissue lysate 4000ug.
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Background
GFER(FAD-linked sulfhydryl oxidase) is also named as ALR, HERV1, HPO. It plays an important role in the disulfide relay system (DRS) in human mitochondria.The GFER gene codes for 2 distinct isoforms that are probably synthesized from the same mRNA with the use of different initiation codons. The long isoform (205 amino acids, 23 kD) is located mainly in the mitochondrial intermembrane space and exists under nonreducing and nondenaturing conditions as a homodimer and a heterodimer. The shorter isoform (125 amino acids, 15 kD), which lacks 80 amino acids at its N terminus compared to the longer isoform, is present predominantly in the nucleus(PMID:19409522).
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References
- Teng EC, Todd LR, Ribar TJ. Gfer inhibits Jab1-mediated degradation of p27kip1 to restrict proliferation of hematopoietic stem cells. Molecular biology of the cell. 22(8):1312-20. 2011.
- Di Fonzo A, Ronchi D, Lodi T. The mitochondrial disulfide relay system protein GFER is mutated in autosomal-recessive myopathy with cataract and combined respiratory-chain deficiency. American journal of human genetics. 84(5):594-604. 2009.
- Huang R, Zhao L, Chen H. Megakaryocytic differentiation of K562 cells induced by PMA reduced the activity of respiratory chain complex IV. PloS one. 9(5):e96246. 2014.
- Balogh T, Lőrincz T, Stiller I, Mandl J, Bánhegyi G, Szarka A. The Level of ALR is Regulated by the Quantity of Mitochondrial DNA. Pathology oncology research : POR. 2015.
- Kumar S, Wang J, Rani R, Gandhi CR. Hepatic Deficiency of Augmenter of Liver Regeneration Exacerbates Alcohol-Induced Liver Injury and Promotes Fibrosis in Mice. PloS one. 11(1):e0147864. 2016.
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