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Product Name
alpha Actin antibody
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Description
alpha Actin Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human colon tissue, human heart tissue, human skeletal muscle tissue. Positive WB detected in rat skeletal muscle tissue, mouse skeletal muscle tissue. Positive IP detected in mouse skeletal muscle tissue. Observed molecular weight by Western-blot: 40-42 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
ACTA antibody; ACTA1 antibody; ACTA1 skeletal Muscle Actin antibody; actin antibody; Actin antibody; alpha skeletal muscle antibody; Alpha actin 1 antibody; ASMA antibody; CFTD antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of alpha Actin recombinant protein (Accession Number: NM_001100). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with Catalog No:107862(ACTA1 Antibody) at dilution of 1:4000
Immunohistochemistry of paraffin-embedded human colon slide using Catalog No:107862(ACTA1 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human colon slide using Catalog No:107862(ACTA1 Antibody) at dilution of 1:50
IP Result of anti-ACTA1 (IP:Catalog No:107862, 4ug; Detection:Catalog No:107862 1:4000) with mouse skeletal muscle tissue lysate 2200ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:107862(ACTA1 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG
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Background
ACTA1 represents actin, alpha 1, skeletal muscle
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References
- Vattepu R, Yadav R, Beck MR. Actin-induced dimerization of palladin promotes actin-bundling. Protein science : a publication of the Protein Society. 24(1):70-80. 2015.
- Li G, Feng T, Pan W, Shi X, Dai J. DEAD-box RNA helicase DDX3X inhibits DENV replication via regulating type one interferon pathway. Biochemical and biophysical research communications. 456(1):327-32. 2015.
- Xiong W, Yang J, Wang M. Vinexin β Interacts with Hepatitis C Virus NS5A, Modulating Its Hyperphosphorylation To Regulate Viral Propagation. Journal of virology. 89(14):7385-400. 2015.
- Imai R, Asai K, Hanai J, Takenaka M. Transgenic mice overexpressing glia maturation factor-β, an oxidative stress inducible gene, show premature aging due to Zmpste24 down-regulation. Aging. 7(7):486-99. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"