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Product Name
Alpha-2-macroglobulin antibody
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Description
Alpha-2-macroglobulin Rabbit Polyclonal antibody. Positive IHC detected in human hepat℃irrhosis tissue, human ovary tissue, human stomach tissue. Positive IF detected in HepG2 cells. Positive IP detected in HepG2 cells. Positive WB detected in human blood tissue, HepG2 cells, human brain tissue, human serum tissue. Observed molecular weight by Western-blot: 185 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
A2M antibody; Alpha 2 M antibody; alpha 2 macroglobulin antibody; CPAMD5 antibody; DKFZp779B086 antibody; FWP007 antibody; S863 7 antibody; α-2M antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Alpha-2-macroglobulin recombinant protein (Accession Number: NM_001347423). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:2000-1:20000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:50-1:500
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Validations
human blood tissue were subjected to SDS PAGE followed by western blot with Catalog No:107877(A2M antibody) at dilution of 1:6000
IP Result of anti-A2M (IP:Catalog No:107877, 5ug; Detection:Catalog No:107877 1:300) with HepG2 cells lysate 2800ug.
Immunohistochemistry of paraffin-embedded human hepatocirrhosis tissue slide using Catalog No:107877(A2M Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human hepatocirrhosis tissue slide using Catalog No:107877(A2M Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HepG2 cells using Catalog No:107877(A2M Antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
alpha-2-macroglobulin, also known as α2-macroglobulin (α2M or A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer’s disease.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"