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Product Name
Aldolase C antibody
- Documents
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Description
Aldolase C Rabbit Polyclonal antibody. Positive WB detected in mouse cerebellum tissue, human heart tissue, K-562 cells, mouse brain tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 40kd
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ALDOC antibody; Aldolase C antibody; Brain type aldolase antibody; Fructose bisphosphate aldolase C antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Aldolase C recombinant protein (Accession Number: NM_005165). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:10-1:100
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Validations
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with Catalog No:107978(ALDoc antibody) at dilution of 1:800
Immunofluorescent analysis of HepG2 cells, using ALDoc antibody Catalog No:107978 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
Fructose-bisphosphate aldolase C (ALDoc) reversibly cleaves FBP and F1-P to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate and is strongly expressed in mammalian brain together with ALDOA and is alsopresent in the heart and spleen of some species(PMID:9363598). It is involed in glycolysis as an important enzyme. Phospholipase D2 and inositol 1,4,5-triphosphate interact with ALDoc in signal transduction. Meanwhile, the protein expression of ALDoc has been reported to be regulated in brain tumor, hepatomas, and lung cancer(PMID:21548097).
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References
- Zhao W, Jiang B, Hu H. Lack of CUL4B leads to increased abundance of GFAP-positive cells that is mediated by PTGDS in mouse brain. Human molecular genetics. 24(16):4686-97. 2015.
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