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Product Name
ALDOB antibody
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Description
ALDOB Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue. Positive IF detected in HepG2 cells. Positive WB detected in mouse kidney tissue, mouse liver tissue, mouse skeletal muscle tissue. Positive IP detected in mouse kidney tissue. Observed molecular weight by Western-blot: 35-40 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ALDB antibody; ALDOB antibody; Liver type aldolase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ALDOB recombinant protein (Accession Number: BC029399). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:107977(ALDOB antibody) at dilution of 1:4000
Immunohistochemical of paraffin-embedded human liver using Catalog No:107977(ALDOB antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:107977(ALDOB antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using ALDOB antibody Catalog No:107977 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-ALDOB (IP:Catalog No:107977, 4ug; Detection:Catalog No:107977 1:1000) with mouse kidney tissue lysate 4000ug.
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Background
Fructose-1,6-bisphosphate aldolase is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate.Vertebrates have 3 aldolase isozymes, aldolase A , B(ALDOB), and C. Deficiency of this enzyme ALDOB causes an accumulation of fructose-1-phosphate after fructose intake, which results in toxic symptoms like vomiting, hypoglycemia, jaundice, elevated liver enzymes and hepatomegaly(PMID:22375183).
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References
- Sun L, Song L, Wan Q. cMyc-mediated activation of serine biosynthesis pathway is critical for cancer progression under nutrient deprivation conditions. Cell research. 25(4):429-44. 2015.
- Tao QF, Yuan SX, Yang F. Aldolase B inhibits metastasis through Ten-Eleven Translocation 1 and serves as a prognostic biomarker in hepatocellular carcinoma. Molecular cancer. 14:170. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"