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Product Name
Albumin antibody
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Description
Albumin Mouse Monoclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human liver tissue. Positive IP detected in HepG2 cells. Positive WB detected in human plasma tissue, human liver tissue. Observed molecular weight by Western-blot: 66 kDa
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Tested applications
ELISA, IHC, IF, WB, IP
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Species reactivity
Human; other species not tested.
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Alternative names
Alb antibody; albumin antibody; Serum albumin antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of Albumin recombinant protein (Accession Number: NM_000477). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:5000-1:50000
IP: 1:2000-1:20000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human liver using Catalog No:107569(ALB antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:107569(ALB antibody) at dilution of 1:50 (under 40x lens)
human plasma (diluted 5000 fold) was subjected to SDS PAGE followed by western blot with Catalog No:107569 (Albumin Antibody) at dilution of 1:10000
IP Result of anti-Albumin (IP:Catalog No:107569, 5ug; Detection:Catalog No:107569 1:5000) with HepG2 cells lysate 1800ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:107569(Albumin Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071)
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References
- Collins MA, An J, Peller D, Bowser R. Total protein is an effective loading control for cerebrospinal fluid western blots. Journal of neuroscience methods. 251:72-82. 2015.
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