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Product Name
AK2 antibody
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Description
AK2 Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in HeLa cells, HEK-293 cells, human heart tissue, human liver tissue, Jurkat cells, Raji cells. Positive IF detected in A549 cells. Positive IHC detected in human pancreas cancer tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human testis tissue. Observed molecular weight by Western-blot: 26 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat,Zebrafish; other species not tested.
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Alternative names
adenylate kinase 2 antibody; ADK2 antibody; AK 2 antibody; AK2 antibody; ATP AMP transphosphorylase 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AK2 recombinant protein (Accession Number: NM_001625). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107923(AK2 antibody) at dilution of 1:800
IP Result of anti-AK2 (IP:Catalog No:107923, 3ug; Detection:Catalog No:107923 1:800) with mouse liver tissue lysate 12500ug.
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:107923(AK2 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of A549 cells, using AK2 antibody Catalog No:107923 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
AK2(Adenylate kinase 2, mitochondrial ) is also named as ADK2 and belongs to the adenylate kinase family. It plays a unique role in energy metabolism and energy transfer by regulating the ATP/ADP rate between the cytoplasma matrix and the mitochondria and it is increased expression during adipocyte and B cell differentiation(PMID:23020757). AK2 catalyzes the reversible transfer of a phosphoryl group from ATP to AMP(PMID:19043417). Defects in AK2 are the cause of reticular dysgenesis (RDYS)(PMID:19043416). It has 6 isoforms produced by alternative splicing.
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References
- Pannicke U, Hönig M, Hess I. Reticular dysgenesis (aleukocytosis) is caused by mutations in the gene encoding mitochondrial adenylate kinase 2. Nature genetics. 41(1):101-5. 2009.
- Fustin JM, Doi M, Yamada H, Komatsu R, Shimba S, Okamura H. Rhythmic nucleotide synthesis in the liver: temporal segregation of metabolites. Cell reports. 1(4):341-9. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"