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Product Name
AK2 antibody
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Description
AK2 Mouse Monoclonal antibody. Positive IHC detected in human liver tissue, human heart tissue. Positive IF detected in HepG2 cells, HeLa cells. Positive FC detected in HepG2 cells. Positive WB detected in HeLa cells, HEK-293 cells. Observed molecular weight by Western-blot: 29 kDa
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human; other species not tested.
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Alternative names
adenylate kinase 2 antibody; ADK2 antibody; AK 2 antibody; AK2 antibody; ATP AMP transphosphorylase 2 antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of AK2 recombinant protein (Accession Number: NM_001625). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107565(AK2 antibody) at dilution of 1:4000
Immunohistochemistry of paraffin-embedded human liver slide using Catalog No:107565(AK2 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human liver slide using Catalog No:107565(AK2 Antibody) at dilution of 1:50
Immunofluorescent analysis of HepG2 cells using Catalog No:107565(AK2 Antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG
1X10^6 HepG2 cells were stained with .2ug AK2 antibody (Catalog No:107565, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.
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Background
AK2(Adenylate kinase 2, mitochondrial ) is also named as ADK2 and belongs to the adenylate kinase family. It plays a unique role in energy metabolism and energy transfer by regulating the ATP/ADP rate between the cytoplasma matrix and the mitochondria and it is increased expression during adipocyte and B cell differentiation(PMID:23020757). AK2 catalyzes the reversible transfer of a phosphoryl group from ATP to AMP(PMID:19043417). Defects in AK2 are the cause of reticular dysgenesis (RDYS)(PMID:19043416). It has 6 isoforms produced by alternative splicing.
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