-
Product Name
AHR antibody
- Documents
-
Description
AHR Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells, HEK-293 cells, MCF-7 cells, NIH/3T3 cells, PC-3 cells. Positive IP detected in PC-3 cells. Positive IHC detected in human brain tissue. Observed molecular weight by Western-blot: 105kd
-
Tested applications
ELISA, WB, IHC, IP
-
Species reactivity
Human, Mouse; other species not tested.
-
Alternative names
Ah receptor antibody; AHR antibody; aryl hydrOCarbon receptor antibody; bHLHe76 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of AHR recombinant protein (Accession Number: NM_001621). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
-
Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:107846(AHR antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human brain using Catalog No:107846(AHR antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-AHR (IP:Catalog No:107846, 4ug; Detection:Catalog No:107846 1:500) with PC-3 cells lysate 1880ug.
-
Background
The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter.
-
References
- Feng XC, Du X, Chen S. Swertianlarin, isolated from Swertia mussotii Franch, increases detoxification enzymes and efflux transporters expression in rats. International journal of clinical and experimental pathology. 8(1):184-95. 2015.
- Chai J, Feng X, Zhang L. Hepatic expression of detoxification enzymes is decreased in human obstructive cholestasis due to gallstone biliary obstruction. PloS one. 10(3):e0120055. 2015.
- Chai J, Du X, Chen S. Oral administration of oleanolic acid, isolated from Swertia mussotii Franch, attenuates liver injury, inflammation, and cholestasis in bile duct-ligated rats. International journal of clinical and experimental medicine. 8(2):1691-702. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"