Product Name

Aggrecan antibody

Documents

Description

Aggrecan Rabbit Polyclonal antibody. Positive IHC detected in human lung tissue. Positive WB detected in ROS1728 cells. Observed molecular weight by Western-blot: 70 kDa

Tested applications

ELISA, WB, IHC

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

ACAN antibody; AGC1 antibody; AGCAN antibody; aggrecan antibody; CSPG1 antibody; CSPGCP antibody; MSK16 antibody; SEDK antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of Aggrecan recombinant protein (Accession Number: BC036445). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IHC: 1:20-1:200

Validations

ROS1728 cells were subjected to SDS PAGE followed by western blot with Catalog No:107829(ACAN Antibody) at dilution of 1:600

Immunohistochemistry of paraffin-embedded human lung tissue slide using Catalog No:107829(ACAN Antibody) at dilution of 1:400 (under 10x lens)

Immunohistochemistry of paraffin-embedded human lung tissue slide using Catalog No:107829(ACAN Antibody) at dilution of 1:400 (under 40x lens)

Background

Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of extracellular matrix of cartilagenous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. This polyclonal antibody raised against 1-298aa of human aggrecan reveals a band of 70 kDa, which is consistent with the molecular weight of degradation product generated by proteolysis at the aggrecanase site (PMID: 7852317; 9337874).

Aggrecan antibody

Product Information

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