Product Name

ADH7 antibody

Documents

Description

ADH7 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A549 cells, HEK-293 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 45-47 kDa

Tested applications

ELISA, WB, IF, IHC

Species reactivity

Human; other species not tested.

Alternative names

ADH 4 antibody; ADH7 antibody; Gastric alcohol dehydrogenase antibody; Retinol dehydrogenase antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of ADH7 recombinant protein (Accession Number: BC131512). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IHC: 1:20-1:200

IF: 1:20-1:200

Validations

HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107790(ADH7 antibody) at dilution of 1:1000

Immunohistochemistry of paraffin-embedded human liver slide using Catalog No:107790(ADH7 Antibody) at dilution of 1:50

Immunofluorescent analysis of HepG2 cells, using ADH7 antibody Catalog No:107790 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Background

ADH7(Alcohol dehydrogenase class 4 mu/sigma chain) is also named as gastric alcohol dehydrogenase, retinol dehydrogenase and belongs to the zinc-containing alcohol dehydrogenase family. The ubiquitous expression pattern of ADH7 during embryogenesis has led to the notion that the enzymatic conversion of retinol to retinal occurs more or less uniformly and plays no role in the spatial or temporal regulation of RA synthesis during embryonic development(PMID:17473173). It has 2 isoforms produced by alternative splicing and can exsit as a homodimer(PMID:18479436).

ADH7 antibody

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