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Product Name
Adenylosuccinate lyase antibody
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Description
Adenylosuccinate lyase Rabbit Polyclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human liver cancer tissue. Positive FC detected in HeLa cells. Positive WB detected in HeLa cells, HepG2 cells. Observed molecular weight by Western-blot: 55 kDa
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Adenylosuccinase antibody; adenylosuccinate lyase antibody; ADSL antibody; AMPS antibody; ASASE antibody; ASL antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Adenylosuccinate lyase recombinant protein (Accession Number: NM_000026). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107785(ADSL antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:107785(ADSL antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:107785(ADSL antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using ADSL antibody Catalog No:107785 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
1X10^6 HeLa cells were stained with 0.2ug ADSL antibody (Catalog No:107785, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
ADSL(adenylosuccinate lyase) is also named as AMPS, ASase, ASL and belongs to the lyase 1 family. It is an enzyme involved in 2 pathways of purine nucleotide metabolism and catalyzes cleavage of succinyl groups to yield fumarate(PMID:18524658). Defects in ADSL are the cause of adenylosuccinase deficiency (ADSL deficiency). In humans, mutations in ADSL lead to an inborn error of metabolism originally characterized by developmental delay, often with autistic features(PMID:20884265)..The ADSL enzymatic activity is reduced in lymphocytes and red blood cells of the patient with severe psychomotor retardation(PMID:9545543). It has 2 isoforms produced by alternative splicing.
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