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Product Name
Human ADSL / Adenylosuccinate Lyase (His Tag) recombinant protein
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Description
Catalyzes two non-sequential steps in de novo AMP synthesis: converts (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate (SAICAR) to fumarate plus 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide, and thereby also contributes to de novo IMP synthesis, and converts succinyladenosine monophosphate (SAMP) to AMP and fumarate.
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Protein name
Adenylosuccinate lyase
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Protein short names
OTTMUSP00000050234; RP5-1042K10.8; ADL; ASL; ASASE; OTTMUSP00000050233; OTTMUSP00000050230; AMPS
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Uniprot ID
P30566
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Gene Name
ADSL; AMPS
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
A DNA sequence encoding the human ADSL (P30566-1) (Met 1-Leu 484) was expressed, with a polyhistidine tag at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human ADSL comprises 500 amino acids and has a predicted molecular mass of 57 kDa. It migrates as an approximately 53 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Validations
Human ADSL / Adenylosuccinate Lyase Protein (His Tag) SDS-PAGE
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