Product Name

ACTN4 antibody

Documents

Description

ACTN4 Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive IHC detected in human breast cancer tissue, human lung cancer tissue. Positive IF detected in MCF-7 cells, Hela cells. Positive WB detected in HepG2 cells, HeLa cells, HT-1080 cells, MCF7 cells, mouse liver tissue, mouse skeletal muscle tissue, NIH/3T3 cells, rat skeletal muscle tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 100-105 kDa

Tested applications

ELISA, WB, IHC, IF, IP, FC

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

ACTININ 4 antibody; actinin antibody; alpha 4 antibody; ACTN4 antibody; Alpha actinin 4 antibody; DKFZp686K23158 antibody; F actin cross linking protein antibody; FSGS antibody; FSGS1 antibody; Non muscle alpha actinin 4 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of ACTN4 recombinant protein (Accession Number: NM_004924). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:500-1:5000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107712(ACTN4 antibody) at dilution of 1:500

Immunofluorescent analysis of MCF-7 cells, using ACTN4 antibody Catalog No:107712 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:107712(ACTN4 antibody) at dilution of 1:100 (under 10x lens)

1X10^6 HeLa cells were stained with 0.2ug ACTN4 antibody (Catalog No:107712, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

IP Result of anti-ACTN4 (IP:Catalog No:107712, 5ug; Detection:Catalog No:107712 1:1000) with HeLa cells lysate 3000ug.

Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:107712(ACTN4 antibody) at dilution of 1:100 (under 40x lens)

Background

ACTN4 (alpha actinin 4) is a ubiquitous actin-binding protein that cross-links actin filaments into bundles to form filopodia, and has a crucial role in cytoskeletal organization. ACTN4 is colocalized with actin stress fibers and is dispersed in the cytoplasm and in the nucleus. Increased levels of ACTN4 have been associated with cancer metastasis, indicating its implication in cancer cell migration and metastasis. Defects in ACTN4 cause glomerular disease. This antibody recognizes 100-105 kDa of endogenous ACTN4 protein in various lysates. (22370640, 10700177)

References

Agarwal N, Adhikari AS, Iyer SV, Hekmatdoost K, Welch DR, Iwakuma T. MTBP suppresses cell migration and filopodia formation by inhibiting ACTN4. Oncogene. 32(4):462-70. 2013.
Bai C, Tang S, Bai C, Chen X. Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma. Amino acids. 46(4):841-52. 2014.
Hodges JL, Vilchez SM, Asmussen H, Whitmore LA, Horwitz AR. α-Actinin-2 mediates spine morphology and assembly of the post-synaptic density in hippocampal neurons. PloS one. 9(7):e101770. 2014.
Wang MC, Chang YH, Wu CC. Alpha-actinin 4 is associated with cancer cell motility and is a potential biomarker in non-small cell lung cancer. Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer. 10(2):286-301. 2015.

ACTN4 antibody

Product Information

Related Products / Services